Tricine-based buffer improves small-protein resolution and reduces distorted lanes, giving gradient-like separation on nongradient gels. Dilute 10X to 1X and run at 150-250 V. Recommended with Affinibody one-step PAGE gel kits.
| Name | Catalog No. | Size | Price (RMB) |
| High-Resolution SDS-PAGE Running Buffer (10X) | AFHR-1000 | 1000 ml | 258 |
This tricine-based running buffer replaces glycine to reduce lane distortion and improve separation of small proteins, giving gradient-like resolution on nongradient gels. Run at constant 150–250 V. Dilute the 10X concentrate to 1X with ddH2O. Recommended with Affinibody stain-free or conventional one-step PAGE gel preparation kits.